Ayurveda is oldest science of treatment. In ancient era, there are many plants which are easily available were used in treatment in different forms. As years passed, due to various reasons many plants didn’t survived till date. Some useful important species already extinct and many having fear of extinct in near future. This can cause huge loss to authentic ayurvedic treatment protocols.Tissue culture is modern technique to replicate the plant with the help of single cell. Different methods of tissue culture can actually assure us about rare plants conservation.